Cell Line Authentication Database

Browse our resources to explore the current best practices for validating storing and handling biological materials and how these techniques support data integrity and reproducibility.

Cell line authentication database. Cosmic the catalogue of somatic mutations in cancer is the world s largest and most comprehensive resource for exploring the impact of somatic mutations in human cancer. Among the important resources is the database of cross contaminated or misidentified cell lines 6 maintained by the international cell line authentication committee iclac a group founded in. Using standardized techniques authentication enables communication among all users about the resource and ensures valid reproducible experimental results. Cell lines project v92 released 27 aug 20.

Please be aware that each online cell line reference database uses their own calculations to determine the percentage match. The register currently lists 552 cell lines. Atcc is committed to improving the authentication of cell lines and microbial cultures. Both incorrectly identified animal and human lines have been distributed repeatedly thus invalidating years of hard work.

Cell line authentication research services. Its main feature is cell line identification according to the standard ansi atcc asn 0002 2011 but it also provides features for searching profiles by. Cell line authentication is required whenever a new cell line is established. 509 cell lines are misidentified with no known authentic stock and are listed in table 1.

Authentication of a cell line is the sum of the process by which a line s identity is verified and shown to be free of contamination from other cell lines and microbes. An effort has been made to exclude cell lines that legitimately come from the same donor for example if one cell line was intentionally derived from another. To address this issue a growing number of journals and funding agencies now require researchers to authenticate their cell lines using an accepted consensus method such as short tandem repeat str profiling. It should also be conducted before freezing at any time when the line is inconsistent.

Please ask your labcorp representative for more information on the calculations labcorp uses for comparing human. The cell line integrated molecular authentication clima database is the result of an integration effort aimed at including all certified str profiles of human cell lines in a unique database. Consequently data derived from misidentified cell lines can be misleading or false and cause confusion in the scientific literature. Online reference databases cell line str profiles.